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QSAR predictions:

a) Predictions generated by existing QSARs and expert rule-based systems implemented in different software, both commercial and freeware

b) Use of different tools to apply a consensus approach and thus enhance the reliability of the predictions

c) Assessment of the each prediction reliability, as required by OECD principles for the validation, for regulatory purposes, of QSARs

d) Detailed report compiled according to the information required by the OECD

Read-across and grouping analysis:

a) Structural analogue search and identification of potential candidates for the read-across

b) Checking the availability of toxicological and eco-toxicological experimental data for the candidate substance in the read-across workflow

c) Read-across prediction for the target chemical (weighing the experimental data according to their relevance/reliability)

d) Detailed documentation of the read-across study in line with the corresponding guidelines published by ECHA

Endpoints

a) PHYSICO-CHEMICAL PROPERTIES

7.2 MELTING/FREEZING POINT

7.3 BOILING POINT

7.4 RELATIVE DENSITY

7.5 VAPOUR PRESSURE

7.6 SURFACE TENSION

7.7 WATER SOLUBILITY

7.8 PARTITION COEFFICIENT n-Octanol/Water

7.9 FLASH POINT

7.16 DISSOCIATION CONSTANT

b) TOXICOLOGICAL PROPERTIES

8.1 SKIN IRRITATION or SKIN CORROSION

8.2 EYE-IRRITATION

8.3 SKIN SENSITIZATION

8.4 MUTAGENICITY

8.4.1 IN-VITRO GENE MUTATION STUDY IN BACTERIA

8.4.2 IN-VITRO CITOGENICITY STUDY IN MAMMALIAN CELLS OR IN-VITRO MICRONUCLEUS STUDY

8.4.4 IN-VIVO MUTAGENICITY STUDY

8.5 ACUTE TOXICITY

8.5.1 ACUTE TOXICITY (by oral route)

8.7 REPRODUCTIVE TOXICITY

8.7.1 SCREENING FOR REPRODUCTIVE/DEVELOPMENTAL TOXICITY

8.7.2 PRE-NATAL DEVELOPMENTAL TOXICITY STUDY

8.9 CARCINOGENICITY

c) ECOTOXICOLOGICAL AND PROPERTIES ENVIRONMENTAL PROPERTIES

9.1 AQUATIC TOXICITY

9.1.1 SHORT-TERM TOXICITY TESTING ON INVERTEBRATES (DAPHNIA)

9.1.2 GROWTH INHIBITION STUDY ON AQUATIC PLANT (ALGAE)

9.1.3 SHORT-TERM TOXICITY TESTING ON FISH

9.1.5 LONG-TERM TOXICITY TESTING ON INVERTEBRATES (DAPHNIA)

9.1.6 LONG-TERM TOXICITY TESTING ON FISH

9.2 DEGRADATION

9.2.1 BIOTIC

9.2.1.1 READY BIODEGRADABILITY

9.2.1.3 SOIL SIMULATION TESTING

9.2.2 ABIOTIC

9.2.2.1 HYDROLYSIS AS A FUNCTION OF pH

9.3 FATE AND BEHAVIOUR IN THE ENVIRONMENT

9.3.1 ABSORPTION/DESORPTION SCREENING

9.3.2 BIOACCUMULATION IN AQUATIC SPECIES, PREFERABLE FISH

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